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KMID : 1003819990030020053
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Journal Of The Korea Institute Of Oriental Medical Diagnostics
1999 Volume.3 No. 2 p.53 ~ p.64
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Effects of Acupuncture and Radix Astragali Aqua-acupuncture at Synsu(BL23) on Theranscriptional Expression of Mouse Cytokine IFN-γ
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Abstract
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Interferons(IFNs) are a family of proteins that shara the property of interfering with the replication of viruses, these are three main types, IFN-α, IFN-β, and IFN-γ. Especially, IFN-γ("immuno interferon") is secreted by antigen- or mitogen- stimulated lymphocytes. It stimulates the expression of class Ⅱ MHC moelcules on macrophages and also activates them to enhanced killing of interacellular bacteria and tumor cell.
Radix Astragali is used (1) to replenish the vital energy and to stop perspiration(anhidrotic) for the treatment of spontaneous perspiration, night sweat, prolapse of uterus and anus; (2) to dispel pus and accelerating the healing of wounds for the treatment of chronic ulcer; (3) as a diuretic for chronic nephritis with edema and proteinuria.
Aqua-acupuncture treatment is composed of acupuncture-treatment and herbal drugs´ action. Acupuncture-treatment is known as using by the physiological function of the Channels and Collaterals, pathological changes, induction, transmission and regulation. The channels induce stimulation and transmit information. If some region of the human body is stimulated, such stimulation will be transmitted along the channel to the reated viscera or bowels in the body. As a result, the relevant physiological or pathological changes take place. These changes are reflected on the body surface through the channels. Especially, indications of Synsu(BL23) are nephritis, impotence, irregular menstruation, chronic diarrhea, lumbago.
Acpuncture and Radix Astragali aqua-acupuncture stimuli have been used to cure human diseases. However, it still remains to be unknown on its action mechanism, physiolosical and biochemical aspects. Thus, many attempts were made to show the scientific background covering the above mentioned mechanisms. Most recent studies show that these tests improve blood circulatory system and increase leucocyte counts.
In this study, we have applied the acupuncture stimuli to mouse Sinsu(BL-23), which is a stimulative point of oriental medicine, to see if cytokine such as IFN-γ can be detected. Mice were treated with lipopolysaccharide(LPS) for inflammation induction, and then reverse transcriptase-polymerase chain reaction (RT-PCR) using each primer set and Western Blotting analysis were performed to trace the amounts of mRNA and proteins.
In conclusion, the expression of IFN-γ after treatment of LPS was decreased more than that of before treatment of LPS. Additionally, the efficiency of increased immunity by acupuncture stimuli to mouse Sinsu(BL-23) and Radix Astragali aqua-acupuncture stimuli was also increased. Based on the result, we suggested that the trace of IFN-γ showed the basement of the efficiency by acupuncture stimuli and Radix Astragali aqua-acupuncture stimuli, and could be used to diagnose the processing of the diseases by the immunoreaction of the body.
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